A quick RNA mini-prep for Neurospora mycelial cultures

-prep RNA probed with ccg-1 DNA fragment. Total RNA from a series of transformants into bd A and frq7 A was examined for the presence of the ccg-1 gene transcript (Loros et al. 1989 Science 243:385-388). Each lane contains 10 µg of RNA (1/200

Fast and reliable mini-prep RNA extraction from Neurospora crassa

conditions and strains. From a single colony we could isolate up to 5 µg total RNA.

Mycelium from a liquid or surface grown culture was harvested, washed, dried thoroughly with filter paper and frozen in liquid nitrogen. Single colonies including agar

Single-Cell cDNA Library Construction Using Cycling aRNA Amplification

to maintain the completeness of a cDNA library, resulting in a loss of rare cDNAs. However, gene expression analysis of specific cell populations within a heterogeneous tissue is essential for research in vivo, requiring a method to generate cDNA libraries